Journal: Advanced Science

Article Title: Single‐Cell Morphomechanics of Prostate Cancer‐Associated Fibroblasts Identifies Distinct Features Associated with Patient Outcome

doi: 10.1002/advs.202522440

Figure Lengend Snippet: Modulating TGF‐β signaling changes the morphological and biomechanical features of CAFs and NPFs. a, b) Representative confocal microscopy images of CAFs (a) and NPFs (b) stained for F‐actin, αSMA and nuclei after 48hrs treatment with 10ng mL −1 TGF‐β1 or the TGF‐β inhibitor A83‐01 (and vehicle controls). c, d) Half‐violin plots showing distributions and medians per patient after quantitative analysis of nuclear area (c), and nuclear circularity (d) for three representative patients. Note that F‐actin alignment is not presented due to varying cell densities upon TGF‐β manipulations. e) Median apparent Young's moduli obtained by AFM indentation tests on adherent CAF/NPFs for three patient pairs in dependence on TGF‐β1 and A83‐01 treatment (compared to vehicle controls). f) Representative contour plots from RT‐DC analysis of suspended CAFs and NPFs, showing vehicle controls and cells treated with TGF‐β1 or A83‐01. g, h) Median apparent Young's moduli (h) and volumes i) obtained by RT‐DC for three patient pairs. (c–e, g, h) Results from a linear mixed model statistical analysis are shown ( * p < 0.05, ** p < 0.01, *** p < 0.001). Different donors ( n = 4–5) are highlighted by symbols/patient numbers. See summary of statistical results in Table (Supporting Information).

Article Snippet: A83‐01 , Tocris Bioscience , 5 μ m , , 48 h.

Techniques: Confocal Microscopy, Staining